miRNA-92a-3p regulates osteoblast differentiation in patients with concomitant limb fractures and TBI via IBSP/PI3K-AKT inhibition

miRNA-92a-3p regulates osteoblast differentiation in patients with concomitant limb fractures and TBI via IBSP/PI3K-AKT inhibition

miRNA-92a-3p regulates osteoblast differentiation in patients with concomitant limb fractures and TBI via IBSP/PI3K-AKT inhibition

Sufferers who maintain concomitant fractures and traumatic mind harm (TBI) are identified to have considerably faster fracture-healing charges than sufferers with remoted fractures. The mechanisms underlying this phenomenon have but to be recognized. Within the current examine, we discovered that the upregulation of microRNA-92a-3p (miRNA-92a-3p) induced by TBI correlated with a lower in integrin binding sialoprotein (IBSP) expression in callus formation. In vitro, overexpressing miRNA-92a-3p inhibited IBSP expression and accelerated osteoblast differentiation, whereas silencing of miRNA-92a-3p inhibited osteoblast exercise.
A lower in IBSP facilitated osteoblast differentiation through the Phosphatidylinositol 3-kinase/threonine kinase 1 (PI3K/AKT) signaling pathway. By way of luciferase assays, we discovered proof that IBSP is a miRNA-92a-3p goal gene that negatively regulates osteoblast differentiation. Furthermore, the current examine confirmed that pre-injection of agomiR-92a-3p results in elevated bone formation. Collectively, these outcomes point out that miRNA-92a-3p overexpression could also be a key issue underlying the improved fracture therapeutic noticed in TBI sufferers. Upregulation of miRNA-92a-3p could due to this fact be a promising therapeutic technique for selling fracture therapeutic and stopping nonunion.

EZH2-miRNA Optimistic Suggestions Promotes Tumor Progress in Ovarian Most cancers

Enhancer of zester homolog 2 (EZH2), a histone methyl transferase that mediates H3K27me3 via polycomb repressive complicated 2 (PRC2), is overexpressed in ovarian most cancers and promotes malignant proliferation. Nevertheless, the underlying mechanism of sustaining excessive EZH2 expression stays elusive. Right here we confirmed that microRNA(miRNA) inhibited EZH2 by binding to the three’-UTR of EZH2 mRNA; conversely, EZH2 can inhibit miRNA expression. We confirmed {that a} suggestions loop exists between EZH2 and miRNA that maintained EZH2 overexpression, thus selling ovarian most cancers proliferation in vivo and in vitro.
We additional explored that EZH2 inhibited miRNA expression via PRC2, as decided by CHIP (chromatin immunoprecipitation), and EZH2 decreased the expression of p21, p53, and RUNX3. These outcomes counsel that EZH2 inhibits the expression of Et-miRNAs (EZH2-targeting miRNAs) via the H3K27me3 pathway, thus forming an EZH2-miRNA constructive suggestions loop that maintains the excessive expression of EZH2 and promotes the malignant proliferation of most cancers cells by regulating the expression of cell proliferation-related proteins.

Extracellular Vesicle-Related miRNAs as a Biomarker for Lung Most cancers in Liquid Biopsy

Extracellular vesicles are cell-derived membranous vesicles which might be secreted into biofluids. Rising proof means that EVs play a vital function within the pathogenesis of many illnesses by transferring proteins, genetic materials, and small signaling molecules between cells. Amongst these molecules, microRNAs (miRNAs), a sort of small noncoding RNA, are one of the vital necessary indicators and are concerned in numerous organic processes. Lung most cancers is likely one of the main causes of cancer-related deaths worldwide.
Early analysis of lung most cancers could assist to scale back mortality and enhance the 5 years survival fee and thereby scale back the related socioeconomic burden. Up to now, EV-miRNAs have been acknowledged as biomarkers of a number of cancers to help in analysis or prognosis. On this assessment, we talk about latest findings and scientific observe for EV-miRNAs of lung most cancers in a number of biofluids, together with blood, bronchoalveolar lavage fluid (BALF), and pleural lavage.

Ultrasensitive Detection of Nasopharyngeal Carcinoma-Associated MiRNA via Garland Rolling Circle Amplification Built-in Catalytic Hairpin Meeting

MiRNA is reported to be carefully associated to nasopharyngeal carcinoma and has the potential to be a biomarker for the early analysis of nasopharyngeal carcinoma. Nevertheless, the detection of miRNAs stays to be improved, given their complexity and low sensitivity. Herein, we suggest right here a novel miRNA detection technique via the combination of garland RCA and CHA. Intimately, the strategy consists of two necessary sign amplification processes.
For the primary sign amplification course of, the goal miRNA may provoke garland RCA after which generate a nicking website on the merchandise with the help of Nb.BbvCI enzymes. Afterward, a CHA course of is induced with a designed H probe via the 2 sign amplification processes; the strategy exhibited a much-improved sensitivity. Eventually, we imagine that this technique is a promising strategy able to being utilized in screening, diagnosing, and prognosticating a number of illnesses.
miRNA-92a-3p regulates osteoblast differentiation in patients with concomitant limb fractures and TBI via IBSP/PI3K-AKT inhibition

Identification of exosome miRNAs in bronchial epithelial cells after PM2.5 continual publicity

Quite a few epidemiological research have demonstrated that continual PM2.5 publicity was related to the lung carcinogenesis with out identified potential mechanisms. Exosomes-derived non-coding RNAs, together with miRNAs, are proposed to play crucial function within the incidence and growth of malignant illnesses. So identification of exosomes-derived miRNAs may assist us to higher perceive the molecular toxicity of PM2.5-induced lung most cancers. Institution continual publicity animal and cell mannequin with PM2.5 was carried out as earlier than. HE staining was used for estimating the histological alternations of lungs in vivo. The expressions of EMT markers in vivo and vitro have been quantified by Western blot. Then the exosomes in cell tradition supernatant have been extracted and the concerned miRNAs have been extracted and sequenced. The totally different expression stage of miRNAs have been verified by RT-PCR.
Persistent PM2.5 publicity induced bronchial epithelial cell atypical hyperplasia and big macrophage infiltration. PM2.5 publicity induce EMT occasion in vivo and vitro indicated as elevated expression of Vimentin and decreased expression of E-cadherin. And 5 passages of PM2.5 stimulation additionally induced the discharge of wealthy and extractable exosomes within the cell tradition supernatant in vitro. By way of sequencing, there have been differentially expressed 36 miRNAs between PM2.5 continual uncovered and management teams with 1.5-fold and higher variations.
Amongst them, there have been 30 exosome-miRNAs upregulated and 6 downregulated expression by PM2.5 publicity. The downregulated expression of miR-29b-2-5p, miR-193b-5p and miR-320c and upregulated expression of miR-100-5p, 125b-5p and unconservative_2_45093 in PM2.5 group have been recognized and reconfirmed by qRT-PCR. Persistent PM2.5 publicity causes bronchial epithelial cells atypical hyperplasia and induces EMT occasion in vivo, and it additionally induce the expression variations of miRNAs in exosome in vitro.

Human Liver Kidney Microsome Autoantibody ELISA Kit

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Human Liver kidney microsome autoantibody ELISA Kit

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Human Liver kidney microsome autoantibody ELISA Kit

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Human Liver kidney microsome autoantibody ELISA Kit

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Human Liver kidney microsome autoantibody ELISA Kit

MBS726147-96StripWells 96-Strip-Wells
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Human microRNA-210(miR-210)ELISA

QY-E05678 96T
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Human liver kidney microsome autoantibody,LKM ELISA Kit

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Human liver kidney microsome autoantibody,LKM ELISA Kit

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Human liver kidney microsome autoantibody,LKM ELISA Kit

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Human liver kidney microsome autoantibody(LKM)ELISA Kit

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Human liver kidney microsome autoantibody(LKM)ELISA Kit

GA-E0583HM-96Tests 96 Tests
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Human liver kidney microsome autoantibody(LKM)ELISA Kit

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EUR 346.8

Human liver kidney microsome autoantibody(LKM)ELISA Kit

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EUR 559.2

Human liver kidney microsome autoantibody(LKM)ELISA Kit

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Human liver kidney microsome autoantibody,LKM ELISA Kit

SL1088Hu 96 Tests
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Human Liver Kidney Microsome Autoantibody (LKM) ELISA Kit

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Human Liver Kidney Microsome Autoantibody (LKM) ELISA Kit

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Human Liver Kidney Microsome Autoantibody (LKM) ELISA Kit

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Human Liver Kidney Microsome Autoantibody (LKM) ELISA Kit

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Human liver kidney microsome autoantibody,LKM ELISA Kit

201-12-0567 96 tests
EUR 528
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Human liver / kidney microsomal autoantibody (LKM) ELISA Kit

MBS263743-10x96StripWells 10x96-Strip-Wells
EUR 3040

Human liver / kidney microsomal autoantibody (LKM) ELISA Kit

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Human liver / kidney microsomal autoantibody (LKM) ELISA Kit

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Human liver / kidney microsomal autoantibody (LKM) ELISA Kit

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Human liver/kidney microsomal autoantibody(LKM) ELISA Kit

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Human liver/kidney microsomal auto antibody(LKM) ELISA Kit

NSL1876Hu 96 wells
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Human microrna-21(mir-21) ELISA Kit

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Human MicroRNA-210 (miR-210) ELISA Kit

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Human MicroRNA-210 (miR-210) ELISA Kit

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EUR 550

Human MicroRNA-210 (miR-210) ELISA Kit

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Human MicroRNA-210 (miR-210) ELISA Kit

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Human liver kidney microsome autoantibody,LKM Ab ELISA KIT

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Human liver kidney microsome autoantibody,LKM Ab ELISA KIT

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Human liver kidney microsome autoantibody,LKM Ab ELISA KIT

E0566Hu-596T 5*96T
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Human liver kidney microsome autoantibody,LKM Ab ELISA KIT

E0566Hu-96wells 96 wells
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Human liver kidney microsome type 1 antibody(IgG) Elisa Kit

EK714243 96 Wells
EUR 0.44

Human liver kidney microsome type 1 antibody(IgG) ELISA Kit

CSB-E09074h-24T 1 plate of 24 wells
EUR 198
Description: Qualitativeindirect ELISA kit for measuring Human liver kidney microsome type 1 antibody (IgG) in samples from serum. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Human liver kidney microsome type 1 antibody(IgG) ELISA Kit

1-CSB-E09074h
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  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Qualitativeindirect ELISA kit for measuring Human liver kidney microsome type 1 antibody(IgG) in samples from serum. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Human liver kidney microsome type 1 antibody,IgG ELISA KIT

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Human liver kidney microsome type 1 antibody, IgG ELISA Kit

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Human liver kidney microsome type 1 antibody, IgG ELISA Kit

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Human Anti-Liver Kidney Microsome Type 1 IgG (LKM1) ELISA Kit

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hsa-miRNome MicroRNA Profiling Kit [Human]

RA660A-1 20 profiles
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Liver Kidney Microsome Type 1 Antibody (IgG) (Human) ELISA Kit (OKCA00483)

OKCA00483 96 Wells
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Description: Description of target: Liver Kidney Microsome Type 1 Antibody (IgG);Species reactivity: Human;Application: ;Assay info: Assay Methodology: Qualitative Reverse Capture ELISA;Sensitivity:

Expression Plasmid of Human microRNA MIR670

SC402151 10 µg Ask for price

Expression Plasmid of Human microRNA MIR676

SC402196 10 µg Ask for price

Expression Plasmid of Human microRNA MIR711

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Expression plasmid for human microRNA LET7B

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Expression plasmid for human microRNA LET7c

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Expression plasmid for human microRNA LET7d

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Expression plasmid for human microRNA LET7e

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Expression plasmid for human microRNA LET7g

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Expression plasmid for human microRNA LET7i

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Expression plasmid for human microRNA MIR17

SC400201 10 µg Ask for price

Expression plasmid for human microRNA MIR21

SC400271 10 µg Ask for price

Expression plasmid for human microRNA MIR22

SC400286 10 µg Ask for price

Expression plasmid for human microRNA MIR25

SC400298 10 µg Ask for price

Expression plasmid for human microRNA MIR28

SC400304 10 µg Ask for price

Expression plasmid for human microRNA MIR31

SC400328 10 µg Ask for price

Expression plasmid for human microRNA MIR32

SC400329 10 µg Ask for price

Expression plasmid for human microRNA MIR93

SC400685 10 µg Ask for price

Expression plasmid for human microRNA MIR95

SC400702 10 µg Ask for price

Expression plasmid for human microRNA MIR96

SC400703 10 µg Ask for price

Expression plasmid for human microRNA MIR98

SC400704 10 µg Ask for price

Expression Plasmid of Human microRNA MIR23c

SC401291 10 µg Ask for price

Expression Plasmid of Human microRNA MIR466

SC401800 10 µg Ask for price

Human Anti-LKM1(Anti-Liver Kidney Microsomal type 1 IgG) ELISA Kit

ELK0814-48T 48T Ask for price
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Anti-LKM1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-LKM1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Anti-LKM1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Anti-LKM1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Human Anti-LKM1(Anti-Liver Kidney Microsomal type 1 IgG) ELISA Kit

ELK0814-96T 96T Ask for price
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Anti-LKM1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-LKM1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Anti-LKM1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Anti-LKM1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Human Anti-LKM1 (Anti-Liver Kidney Microsomal type 1 IgG) ELISA Kit

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Human Anti-LKM1 (Anti-Liver Kidney Microsomal type 1 IgG) ELISA Kit

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Expression Plasmid of Human microRNA MIR4661

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Expression Plasmid of Human microRNA MIR4663

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Expression Plasmid of Human microRNA MIR4664

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Expression Plasmid of Human microRNA MIR4665

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Expression Plasmid of Human microRNA MIR4667

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Expression Plasmid of Human microRNA MIR4668

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Expression Plasmid of Human microRNA MIR4669

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Expression Plasmid of Human microRNA MIR4670

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Expression Plasmid of Human microRNA MIR4671

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Expression Plasmid of Human microRNA MIR4672

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Expression Plasmid of Human microRNA MIR4673

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Expression Plasmid of Human microRNA MIR4674

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Expression Plasmid of Human microRNA MIR4675

SC401818 10 µg Ask for price

Expression Plasmid of Human microRNA MIR4676

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Expression Plasmid of Human microRNA MIR4677

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Expression Plasmid of Human microRNA MIR4678

SC401821 10 µg Ask for price

Expression Plasmid of Human microRNA MIR4680

SC401824 10 µg Ask for price

Expression Plasmid of Human microRNA MIR4681

SC401825 10 µg Ask for price

Expression Plasmid of Human microRNA MIR4682

SC401826 10 µg Ask for price

Expression Plasmid of Human microRNA MIR4683

SC401827 10 µg Ask for price

Expression Plasmid of Human microRNA MIR4684

SC401828 10 µg Ask for price

Expression Plasmid of Human microRNA MIR4685

SC401829 10 µg Ask for price

Expression Plasmid of Human microRNA MIR4686

SC401830 10 µg Ask for price

Expression Plasmid of Human microRNA MIR4687

SC401831 10 µg Ask for price

Expression Plasmid of Human microRNA MIR4688

SC401832 10 µg Ask for price

Expression Plasmid of Human microRNA MIR4689

SC401833 10 µg Ask for price

Expression Plasmid of Human microRNA MIR4690

SC401834 10 µg Ask for price

Expression Plasmid of Human microRNA MIR4691

SC401835 10 µg Ask for price

Expression Plasmid of Human microRNA MIR4692

SC401836 10 µg Ask for price

Expression Plasmid of Human microRNA MIR4693

SC401837 10 µg Ask for price

Expression Plasmid of Human microRNA MIR4694

SC401838 10 µg Ask for price

Expression Plasmid of Human microRNA MIR4695

SC401839 10 µg Ask for price

Expression Plasmid of Human microRNA MIR4696

SC401840 10 µg Ask for price

Expression Plasmid of Human microRNA MIR4697

SC401841 10 µg Ask for price

Expression Plasmid of Human microRNA MIR4698

SC401842 10 µg Ask for price

Expression Plasmid of Human microRNA MIR4699

SC401843 10 µg Ask for price

Expression Plasmid of Human microRNA MIR4700

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Expression Plasmid of Human microRNA MIR4701

SC401845 10 µg Ask for price

Expression Plasmid of Human microRNA MIR4703

SC401846 10 µg Ask for price

Expression Plasmid of Human microRNA MIR4704

SC401847 10 µg Ask for price

Expression Plasmid of Human microRNA MIR4705

SC401848 10 µg Ask for price

Expression Plasmid of Human microRNA MIR4706

SC401849 10 µg Ask for price

Expression Plasmid of Human microRNA MIR4707

SC401850 10 µg Ask for price

Expression Plasmid of Human microRNA MIR4708

SC401851 10 µg Ask for price

Expression Plasmid of Human microRNA MIR4709

SC401852 10 µg Ask for price

Expression Plasmid of Human microRNA MIR4710

SC401853 10 µg Ask for price

Expression Plasmid of Human microRNA MIR4711

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Expression Plasmid of Human microRNA MIR4712

SC401855 10 µg Ask for price

Expression Plasmid of Human microRNA MIR4713

SC401856 10 µg Ask for price

Expression Plasmid of Human microRNA MIR4714

SC401857 10 µg Ask for price

Expression Plasmid of Human microRNA MIR4715

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Expression Plasmid of Human microRNA MIR4716

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Expression Plasmid of Human microRNA MIR4717

SC401860 10 µg Ask for price

Expression Plasmid of Human microRNA MIR4718

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Expression Plasmid of Human microRNA MIR4719

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Expression Plasmid of Human microRNA MIR4720

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Expression Plasmid of Human microRNA MIR4721

SC401864 10 µg Ask for price

Expression Plasmid of Human microRNA MIR4722

SC401865 10 µg Ask for price

Expression Plasmid of Human microRNA MIR4723

SC401866 10 µg Ask for price

Expression Plasmid of Human microRNA MIR4724

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Expression Plasmid of Human microRNA MIR4725

SC401868 10 µg Ask for price

Expression Plasmid of Human microRNA MIR4726

SC401869 10 µg Ask for price

Expression Plasmid of Human microRNA MIR4727

SC401870 10 µg Ask for price

Expression Plasmid of Human microRNA MIR4728

SC401871 10 µg Ask for price

Expression Plasmid of Human microRNA MIR4729

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Expression Plasmid of Human microRNA MIR4732

SC401875 10 µg Ask for price

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SC401878 10 µg Ask for price

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SC401880 10 µg Ask for price

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SC401881 10 µg Ask for price

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Expression Plasmid of Human microRNA MIR4751

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SC401895 10 µg Ask for price

Expression Plasmid of Human microRNA MIR4753

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Expression Plasmid of Human microRNA MIR4754

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Expression Plasmid of Human microRNA MIR4755

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Expression Plasmid of Human microRNA MIR4757

SC401900 10 µg Ask for price

Expression Plasmid of Human microRNA MIR4758

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Expression Plasmid of Human microRNA MIR4759

SC401902 10 µg Ask for price

Expression Plasmid of Human microRNA MIR4760

SC401903 10 µg Ask for price
In the meantime, the recognized differentially expressed exosome-miRNAs could partially affiliate with tumorigenesis. To sum up, the recognized exosome-miRNAs could play function within the growth of lung most cancers induced by continual PM2.5 publicity.

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