Changes in Oviductal Cells and Small Extracellular Vesicles miRNAs in Pregnant Cows

Changes in Oviductal Cells and Small Extracellular Vesicles miRNAs in Pregnant Cows

Changes in Oviductal Cells and Small Extracellular Vesicles miRNAs in Pregnant Cows

Early embryonic growth happens within the oviduct, the place a really perfect microenvironment is offered by the epithelial cells and by the oviductal fluid produced by these cells. The oviductal fluid comprises small extracellular vesicles (sEVs), which by way of their contents, together with microRNAs (miRNAs), can guarantee correct cell communication between the mom and the embryo.
Nonetheless, little is understood concerning the modulation of miRNAs inside oviductal epithelial cells (OECs) and sEVs from the oviductal fluid in pregnant cows. On this research, we consider the miRNAs profile in sEVs from the oviductal flushing (OF-sEVs) and OECs from pregnant cows in comparison with non-pregnant, at 120 h after ovulation induction. In OF-sEVs, eight miRNAs (bta-miR-126-5p, bta-miR-129, bta-miR-140, bta-miR-188, bta-miR-219, bta-miR-345-3p, bta-miR-4523, and bta-miR-760-3p) have been up-regulated in pregnant and one miRNA (bta-miR-331-5p) was up-regulated in non-pregnant cows.
In OECs, six miRNAs (bta-miR-133b, bta-miR-205, bta-miR-584, bta-miR-551a, bta-miR-1193, and bta-miR-1225-3p) have been up-regulated in non-pregnant and none was up-regulated in pregnant cows. Our outcomes recommend that embryonic maternal communication mediated by sEVs initiates within the oviduct, and the passage of gametes and the embryo presence modulate miRNAs contents of sEVs and OECs. Moreover, we demonstrated the transcriptional ranges modulation of chosen genes in OECs in pregnant cows. Due to this fact, the embryonic-maternal crosstalk doubtlessly begins throughout early embryonic growth within the oviduct by way of the modulation of miRNAs in OECs and sEVs in pregnant cows.

Expression of miRNA in weight problems and insulin resistance: an overview

MicroRNAs (miRNAs) are a part of the epigenetic mechanisms that regulate gene expression at a post-transcriptional degree. This overview describes some miRNAs whose expression is modified in weight problems and which may be concerned within the growth of insulin resistance. The metabolic alterations related to weight problems are attributable to an adipose tissue dysfunction. miRNAs are a mechanism that regulates gene expression, one miRNA can regulate the expression as much as a thousand genes, and on the identical time one gene may be regulated by a number of miRNAs; furthermore, miRNA expression is tissue particular.
Weight problems results in a dysregulation of miRNA expression in adipose tissue, and adjustments in miRNA expression relate to adjustments in gene expression associated to the event of insulin resistance. Nonetheless, as a result of miRNA may be exported to the extracellular medium by way of exosomes, proteins, and lipoproteins, miRNA may be present in extracellular fluids like blood, urine, saliva, and cerebrospinal fluid. Contemplating the above, miRNA have been proposed as organic markers of various illnesses, and in addition as potential therapeutic targets.

Bioinformatics evaluation of a TF-miRNA-lncRNA regulatory community in main depressive dysfunction

Main depressive dysfunction (MDD) is a extremely prevalent illness and one of many important causes of incapacity worldwide. Though many research have partially revealed the prevalence and growth strategy of MDD, the pathogeny and molecular mechanisms will not be totally understood. Weighted gene coexpression community evaluation (WGCNA) was used to discover the co-expression modules and hub genes in MDD. A protein-protein interplay (PPI) community of essentially the most vital module and a TF-miRNA-lncRNA regulatory community of MDD have been constructed utilizing bioinformatics evaluation instruments.
Changes in Oviductal Cells and Small Extracellular Vesicles miRNAs in Pregnant Cows
A KEGG pathway and gene ontology (GO) practical enrichment evaluation of the genes within the vital module was carried out utilizing DAVID. 5 hub genes within the PPI community and 10 genes within the TF-miRNA-lncRNA regulatory community with excessive diploma values have been recognized, which can present new insights for the investigation of key pathways, diagnostic bio-markers, and therapeutic targets of MDD. This research brings a novel perspective and supplies helpful info to discover the molecular mechanism of MDD.

Expression profiling of Echinococcus multilocularis miRNAs all through metacestode growth in vitro

The uncared for zoonotic illness alveolar echinococcosis (AE) is attributable to the metacestode stage of the tapeworm parasite Echinococcus multilocularis. MicroRNAs (miRNAs) are small non-coding RNAs with a serious function in regulating gene expression in key organic processes. We analyzed the expression profile of E. multilocularis miRNAs all through metacestode growth in vitro, decided the spatial expression of miR-71 in metacestodes cultured in vitro and predicted miRNA targets. Small cDNA libraries from totally different samples of E. multilocularis have been sequenced. We confirmed the expression of 37 miRNAs in E. multilocularis being a few of them absent within the host, reminiscent of miR-71.
We discovered a couple of miRNAs extremely expressed in all life cycle phases and circumstances analyzed, whereas most miRNAs confirmed very low expression. Essentially the most expressed miRNAs have been miR-71, miR-9, let-7, miR-10, miR-4989 and miR-1. The excessive expression of those miRNAs was conserved in different tapeworms, suggesting important roles in growth, survival, or host-parasite interplay. We discovered extremely regulated miRNAs in the course of the totally different transitions or cultured circumstances analyzed, which could recommend a task within the regulation of developmental timing, host-parasite interplay, and/or in sustaining the distinctive developmental options of every developmental stage or situation.
We decided that miR-71 is expressed in germinative cells and in different cell sorts of the germinal layer in E. multilocularis metacestodes cultured in vitro. MiRNA goal prediction of essentially the most extremely expressed miRNAs and in silico practical evaluation confirmed conserved and important roles for these miRNAs in parasite biology. We discovered related targets doubtlessly concerned in growth, cell progress and demise, lifespan regulation, transcription, sign transduction and cell motility.

Mouse Troponin C, slow skeletal and cardiac muscles, Tnnc1 ELISA
ELI-36461m 96 Tests
EUR 1038

Bovine Troponin C, slow skeletal and cardiac muscles, TNNC1 ELIS
ELI-17222b 96 Tests
EUR 1113.6

Human Troponin C, Slow Skeletal And Cardiac Muscles (TNNC1) ELISA Kit
20-abx153332
  • EUR 8853.60
  • EUR 4719.60
  • EUR 1093.20
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Human Troponin C, Slow Skeletal And Cardiac Muscles (TNNC1) ELISA Kit
abx352187-96tests 96 tests
EUR 943.2

Chicken Troponin C, Slow Skeletal And Cardiac Muscles (TNNC1) ELISA Kit
abx555347-96tests 96 tests
EUR 1093.2

Human Troponin C, Slow Skeletal And Cardiac Muscles (TNNC1) ELISA Kit
abx555435-96tests 96 tests
EUR 943.2

Mouse Troponin C, Slow Skeletal And Cardiac Muscles (TNNC1) ELISA Kit
abx555608-96tests 96 tests
EUR 886.8

Pig Troponin C, Slow Skeletal And Cardiac Muscles (TNNC1) ELISA Kit
abx555672-96tests 96 tests
EUR 1093.2

Cow Troponin C, Slow Skeletal And Cardiac Muscles (TNNC1) ELISA Kit
abx555769-96tests 96 tests
EUR 1093.2

Human TNNC1/ Troponin C, slow skeletal and cardiac muscles ELISA Kit
E2558Hu 1 Kit
EUR 726

ELISA kit for Human Troponin C, slow skeletal and cardiac muscles
EK3670 96 tests
EUR 804
Description: Enzyme-linked immunosorbent assay kit for quantification of Human Troponin C, slow skeletal and cardiac muscles in samples from serum, plasma, tissue homogenates and other biological fluids.

ELISA kit for Chicken Troponin C, slow skeletal and cardiac muscles (TNNC1)
KTE30009-48T 48T
EUR 424.8
Description: Quantitative sandwich ELISA for measuring Chicken Troponin C, slow skeletal and cardiac muscles (TNNC1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Chicken Troponin C, slow skeletal and cardiac muscles (TNNC1)
KTE30009-5platesof96wells 5 plates of 96 wells
EUR 2702.4
Description: Quantitative sandwich ELISA for measuring Chicken Troponin C, slow skeletal and cardiac muscles (TNNC1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Chicken Troponin C, slow skeletal and cardiac muscles (TNNC1)
KTE30009-96T 96T
EUR 686.4
Description: Quantitative sandwich ELISA for measuring Chicken Troponin C, slow skeletal and cardiac muscles (TNNC1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Mouse Troponin C, slow skeletal and cardiac muscles (TNNC1)
KTE70102-48T 48T
EUR 398.4
Description: Quantitative sandwich ELISA for measuring Mouse Troponin C, slow skeletal and cardiac muscles (TNNC1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Mouse Troponin C, slow skeletal and cardiac muscles (TNNC1)
KTE70102-5platesof96wells 5 plates of 96 wells
EUR 2538
Description: Quantitative sandwich ELISA for measuring Mouse Troponin C, slow skeletal and cardiac muscles (TNNC1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Mouse Troponin C, slow skeletal and cardiac muscles (TNNC1)
KTE70102-96T 96T
EUR 646.8
Description: Quantitative sandwich ELISA for measuring Mouse Troponin C, slow skeletal and cardiac muscles (TNNC1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Goat Troponin C, slow skeletal and cardiac muscles (TNNC6)
KTE50026-48T 48T
EUR 510
Description: Quantitative sandwich ELISA for measuring Goat Troponin C, slow skeletal and cardiac muscles (TNNC6) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Goat Troponin C, slow skeletal and cardiac muscles (TNNC6)
KTE50026-5platesof96wells 5 plates of 96 wells
EUR 3631.2
Description: Quantitative sandwich ELISA for measuring Goat Troponin C, slow skeletal and cardiac muscles (TNNC6) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Goat Troponin C, slow skeletal and cardiac muscles (TNNC6)
KTE50026-96T 96T
EUR 907.2
Description: Quantitative sandwich ELISA for measuring Goat Troponin C, slow skeletal and cardiac muscles (TNNC6) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

Custom production of antibodies in 5 Rats using customer supplied antigen (std 63 days protocol)
RAT-5 1
EUR 1365.6

Total Protein Extraction Kit for Muscles (50 tests)
P5A06 - Ask for price

Rat Skeletal Muscle Tissue Lysate
LYSATE0010 200ug
EUR 180
Description: This cell lysate is prepared from rat skeletal muscle tissue using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

rno-miRNome MicroRNA Profiling Kit [Rat]
RA680B-1 20 complete profiles
EUR 2006.4

MicroRNA Isolation Kit
KS341025 1 kit
EUR 307.2
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Rat Skeletal Muscle Whole tissue lysate
RAL-1467 1 mg
EUR 628.8

Skeletal Muscle Lysate
21-107 0.1 mg
EUR 342.6
Description: Bovine skeletal muscle tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The bovine skeletal muscle tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the skeletal muscle tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The skeletal muscle tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.

Skeletal Muscle Lysate
21-197 0.1 mg
EUR 342.6
Description: Monkey (Cynomolgus) skeletal muscle tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The monkey (Cynomolgus) skeletal muscle tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the skeletal muscle tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The skeletal muscle tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.

Myosin antibody (skeletal)
10R-7875 100 ug
EUR 386.4
Description: Mouse monoclonal Myosin antibody

Myosin antibody (skeletal)
10R-7876 100 ug
EUR 386.4
Description: Mouse monoclonal Myosin antibody

Skeletal Muscle Lysate
1467 0.1 mg
EUR 229.2
Description: Skeletal Muscle tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Cancer MicroRNA qPCR Array
RA610A-1 10 profiles
EUR 1406.4

Rat Muscle PrimaCell1: Normal Skeletal Muscle Cells
2-82573 1 Kit Ask for price

Rat Ryanodine Receptor 1, Skeletal (RYR1) Protein
20-abx654972
  • EUR 693.60
  • EUR 309.60
  • EUR 2064.00
  • EUR 828.00
  • EUR 510.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

cDNA from Rat Normal Tissue: Skeletal Muscle
C1434171 40 reactions
EUR 451.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Rat Skeletal muscle Actinin Alpha ELISA kit
E02S0267-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Rat Skeletal muscle Actinin Alpha in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Skeletal muscle Actinin Alpha ELISA kit
E02S0267-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Rat Skeletal muscle Actinin Alpha in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Skeletal muscle Actinin Alpha ELISA kit
E02S0267-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Rat Skeletal muscle Actinin Alpha in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Troponin-I Rat Skeletal Muscle, pure protein
TNIM50-N-25 25 ug
EUR 270

MicroRNA scramble in CD511 - Virus
PMIRH000VA-1 1x10^6 IFUs
EUR 937.2

MicroRNA Precursor Pooled Plasmid Library
PMIRHPLPA-1 1 aliquot plasmid DNA
EUR 60

SanPrep Column microRNA Miniprep Kit
SK8811 50prep
EUR 151.32

Stem Cell MicroRNA Profiling Panel
RA620A-1 10 profiles
EUR 1406.4

Troponin I Skeletal Protein
abx060758-100ug 100 ug
EUR 1888.8

Troponin T Skeletal Protein
abx060767-05mg 0.5 mg
EUR 2055.6

anti-fast skeletal Myosin
YF-PA24194 50 ul
EUR 400.8
Description: Mouse polyclonal to fast skeletal Myosin

anti-fast skeletal Myosin
YF-PA13308 50 ug
EUR 435.6
Description: Mouse polyclonal to fast skeletal Myosin

Skeletal Muscle Cytoplasmic Lysate
XBL-10851 0.1 mg
EUR 273.3
Description: human skeletal muscle tissue cytoplasmic protein lysate was prepared by isolating the cytoplasmic protein from whole tissue homogenates using a proprietary technique. The human skeletal muscle tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The cytoplasmic protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, glycerol, and a cocktail of protease inhibitors. For quality control purposes, the isolated Skeletal Muscle tissue cytoplasmic protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated Skeletal Muscle tissue cytoplasmic protein is then Western analyzed by GAPDH antibody, and the expression level is consistent with each lot.

Skeletal Muscle Membrane Lysate
XBL-10852 0.1 mg
EUR 619.8
Description: human skeletal muscle tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human skeletal muscle tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated Skeletal Muscle tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated Skeletal Muscle tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.

Rat Ryanodine Receptor 1, Skeletal (RYR1) ELISA Kit
20-abx156055
  • EUR 8684.40
  • EUR 4626.00
  • EUR 1074.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Rat Ryanodine Receptor 1, Skeletal (RYR1) CLIA Kit
20-abx493893
  • EUR 10282.80
  • EUR 5472.00
  • EUR 1262.40
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Rat Slow Skeletal Troponin I (TNNI1) CLIA Kit
20-abx494152
  • EUR 10282.80
  • EUR 5472.00
  • EUR 1262.40
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Rat Fast Skeletal Troponin I (TNNI2) CLIA Kit
20-abx494155
  • EUR 10282.80
  • EUR 5472.00
  • EUR 1262.40
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Rat Slow Skeletal Troponin T (TNNT1) CLIA Kit
20-abx494158
  • EUR 10282.80
  • EUR 5472.00
  • EUR 1262.40
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Genomic DNA from Rat Normal Tissue: Skeletal Muscle
D1434171 100 ug
EUR 338.4
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Rat Ryanodine Receptor 1, Skeletal (RYR1) ELISA Kit
DLR-RYR1-Ra-48T 48T
EUR 658.8
Description: A sandwich quantitative ELISA assay kit for detection of Rat Ryanodine Receptor 1, Skeletal (RYR1) in samples from tissue homogenates, cell lysates or other biological fluids.

Rat Ryanodine Receptor 1, Skeletal (RYR1) ELISA Kit
DLR-RYR1-Ra-96T 96T
EUR 861.6
Description: A sandwich quantitative ELISA assay kit for detection of Rat Ryanodine Receptor 1, Skeletal (RYR1) in samples from tissue homogenates, cell lysates or other biological fluids.

Rat Actin, Alpha skeletal muscle(ACTA1) ELISA kit
E02A1218-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Rat Actin, Alpha skeletal muscle(ACTA1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Actin, Alpha skeletal muscle(ACTA1) ELISA kit
E02A1218-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Rat Actin, Alpha skeletal muscle(ACTA1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Actin, Alpha skeletal muscle(ACTA1) ELISA kit
E02A1218-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Rat Actin, Alpha skeletal muscle(ACTA1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Muscle, skeletal, receptor tyrosine kinase ELISA kit
E02M0028-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Rat Muscle, skeletal, receptor tyrosine kinase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Muscle, skeletal, receptor tyrosine kinase ELISA kit
E02M0028-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Rat Muscle, skeletal, receptor tyrosine kinase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Muscle, skeletal, receptor tyrosine kinase ELISA kit
E02M0028-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Rat Muscle, skeletal, receptor tyrosine kinase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Actin Alpha 1, Skeletal Muscle ELISA kit
E02A0391-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Rat Actin Alpha 1, Skeletal Muscle in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Actin Alpha 1, Skeletal Muscle ELISA kit
E02A0391-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Rat Actin Alpha 1, Skeletal Muscle in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Actin Alpha 1, Skeletal Muscle ELISA kit
E02A0391-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Rat Actin Alpha 1, Skeletal Muscle in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Ryanodine Receptor 1, Skeletal (RYR1) ELISA Kit
RD-RYR1-Ra-48Tests 48 Tests
EUR 668.4

Rat Ryanodine Receptor 1, Skeletal (RYR1) ELISA Kit
RD-RYR1-Ra-96Tests 96 Tests
EUR 930

Rat Ryanodine Receptor 1, Skeletal (RYR1) ELISA Kit
RDR-RYR1-Ra-48Tests 48 Tests
EUR 699.6

Rat Ryanodine Receptor 1, Skeletal (RYR1) ELISA Kit
RDR-RYR1-Ra-96Tests 96 Tests
EUR 973.2

Rat Ryanodine Receptor 1, Skeletal (RYR1) ELISA Kit
SEC773Ra-10x96wellstestplate 10x96-wells test plate
EUR 6149.04
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Ryanodine Receptor 1, Skeletal (RYR1) in tissue homogenates, cell lysates and other biological fluids.

Rat Ryanodine Receptor 1, Skeletal (RYR1) ELISA Kit
SEC773Ra-1x48wellstestplate 1x48-wells test plate
EUR 611.57
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Ryanodine Receptor 1, Skeletal (RYR1) in tissue homogenates, cell lysates and other biological fluids.

Rat Ryanodine Receptor 1, Skeletal (RYR1) ELISA Kit
SEC773Ra-1x96wellstestplate 1x96-wells test plate
EUR 822.24
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Ryanodine Receptor 1, Skeletal (RYR1) in tissue homogenates, cell lysates and other biological fluids.

Rat Ryanodine Receptor 1, Skeletal (RYR1) ELISA Kit
SEC773Ra-5x96wellstestplate 5x96-wells test plate
EUR 3340.08
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Ryanodine Receptor 1, Skeletal (RYR1) in tissue homogenates, cell lysates and other biological fluids.

Rat Ryanodine Receptor 1, Skeletal (RYR1) ELISA Kit
4-SEC773Ra
  • EUR 6210.00
  • EUR 3280.80
  • EUR 823.20
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Rat Ryanodine Receptor 1, Skeletal (RYR1) in samples from tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species.

Total RNA from Rat Normal Tissue: Skeletal Muscle
R1434171-50 50 ug
EUR 182.4
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Rat Ryanodine Receptor 1, Skeletal(RYR1)ELISA Kit
QY-E10032 96T
EUR 433.2

Rat Ryanodine Receptor 1, Skeletal ELISA Kit (RYR1)
RK03928 96 Tests
EUR 625.2

OncoMir pooled microRNA precursor virus library
PMIRHOPLVA-1 1 virus aliquot
EUR 1429.2

MicroRNA precursor pooled library, HT prepackaged
PMIRHPLVAHT-1 10 virus aliquots
EUR 12042

Human microRNA-210(miR-210)ELISA
QY-E05678 96T
EUR 433.2

hsa-miRNome MicroRNA Profiling Kit [Human]
RA660A-1 20 profiles
EUR 2212.8

mmu-miRNome MicroRNA Profiling Kit [Mouse]
RA670A-1 20 profiles
EUR 2046

Rat SeraMir Exosome RNA 384 microRNA qPCR Profiler Assay Set
RA812A-1 20 profiles
EUR 634.8

Rabbit Skeletal Muscle Troponin-C
7-06205 1mg Ask for price

Rabbit Skeletal Muscle Troponin-C
7-06206 5mg Ask for price

Rabbit Skeletal Muscle Troponin-C
7-06207 10mg Ask for price

Troponin I antibody (Skeletal Muscle)
10-T126C 1 mg
EUR 562.8
Description: Mouse monoclonal Troponin I antibody (Skeletal Muscle)

Troponin I antibody (Skeletal Muscle)
10-T81B 1 mg
EUR 603.6
Description: Mouse monoclonal Troponin I antibody (Skeletal Muscle)

alpha Actin (Skeletal Muscle) (Rabbit)
30R-3107 1 mg
EUR 308.4
Description: Purified native alpha Actin (Skeletal Muscle) (Rabbit)

alpha Actin (skeletal muscle) Antibody
35512-100ul 100ul
EUR 468

Troponin T protein (Skeletal Muscle)
30R-AT042 100 ug
EUR 969.6
Description: Purified native Human Troponin T protein (Skeletal Muscle)

Troponin I protein (Skeletal Muscle)
30R-AT060 100 ug
EUR 1412.4
Description: Purified native Troponin I protein (Skeletal Muscle)

alpha Actin (Skeletal Muscle) (Mouse)
30R-3408 500 ug
EUR 1701.6
Description: Recombinant Mouse Actin, alpha skeletal muscle(Acta1)

Troponin I antibody (Skeletal Muscle)
10R-T126a 1 mg
EUR 681.6
Description: Mouse monoclonal Troponin I antibody (Skeletal Muscle)

Troponin I antibody (Skeletal Muscle)
10R-T126b 1 mg
EUR 681.6
Description: Mouse monoclonal Troponin I antibody (Skeletal Muscle)

Troponin I antibody (Skeletal Muscle)
10R-T126c 1 mg
EUR 681.6
Description: Mouse monoclonal Troponin I antibody (Skeletal Muscle)

Troponin I protein (Skeletal muscle)
30-AT48 100 ug
EUR 706.8
Description: Purified native Human Troponin I protein (Skeletal muscle)

Troponin I Skeletal Muscle Antibody
abx020870-1mg 1 mg
EUR 978

Troponin I Skeletal Muscle Antibody
abx020871-1mg 1 mg
EUR 978

?-Skeletal Muscle Actin Polyclonal Antibody
ES1176-100ul 100ul
EUR 334.8
Description: A Rabbit Polyclonal antibody against ?-Skeletal Muscle Actin from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA

?-Skeletal Muscle Actin Polyclonal Antibody
ES1176-50ul 50ul
EUR 248.4
Description: A Rabbit Polyclonal antibody against ?-Skeletal Muscle Actin from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA

? skeletal muscle actin Monoclonal Antibody
EM1149-100ul 100ul
EUR 334.8
Description: A Mouse Monoclonal antibody against ? skeletal muscle actin from Human/ Rat/ Mouse. This antibody is tested and validated for WB, ELISA, IHC

? skeletal muscle actin Monoclonal Antibody
EM1149-50ul 50ul
EUR 248.4
Description: A Mouse Monoclonal antibody against ? skeletal muscle actin from Human/ Rat/ Mouse. This antibody is tested and validated for WB, ELISA, IHC

?-Skeletal Muscle Actin Polyclonal Antibody
EA047-100ul 100ul
EUR 334.8
Description: A Rabbit Polyclonal antibody against ?-Skeletal Muscle Actin from Human/ Rat/ Mouse. This antibody is tested and validated for WB, ELISA, IHC

?-Skeletal Muscle Actin Polyclonal Antibody
EA047-50ul 50ul
EUR 248.4
Description: A Rabbit Polyclonal antibody against ?-Skeletal Muscle Actin from Human/ Rat/ Mouse. This antibody is tested and validated for WB, ELISA, IHC

Mouse Skeletal Muscle Tissue Lysate
LYSATE0021 200ug
EUR 180
Description: This cell lysate is prepared from mouse skeletal muscle tissue using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Anti-fast skeletal Myosin (3H3)
YF-MA18264 100 ug
EUR 435.6
Description: Mouse monoclonal to fast skeletal Myosin

Anti-fast skeletal Myosin (1B22)
YF-MA19700 100 ug
EUR 435.6
Description: Mouse monoclonal to fast skeletal Myosin

Skeletal Muscle Diabetic Disease Lysate
XBL-10369 0.1 mg
EUR 796.2
Description: human skeletal muscle tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The human skeletal muscle tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the Skeletal Muscle tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The Skeletal Muscle tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.

Anti-fast skeletal Myosin (2D9)
YF-MA10603 100 ug
EUR 435.6
Description: Mouse monoclonal to fast skeletal Myosin

Rat Troponin I, Slow Skeletal Muscle (TNNI1) ELISA Kit
20-abx156169
  • EUR 8684.40
  • EUR 4626.00
  • EUR 1074.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Rat Troponin I, Fast Skeletal Muscle (TNNI2) ELISA Kit
20-abx156170
  • EUR 8684.40
  • EUR 4626.00
  • EUR 1074.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Rat Troponin T, Slow Skeletal Muscle (TNNT1) ELISA Kit
20-abx156172
  • EUR 8684.40
  • EUR 4626.00
  • EUR 1074.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Rat Troponin I Type 1, Slow Skeletal (TNNI1) Protein
20-abx069487
  • EUR 693.60
  • EUR 309.60
  • EUR 2064.00
  • EUR 828.00
  • EUR 510.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Rat Troponin I Type 2, Fast Skeletal (TNNI2) Protein
20-abx069492
  • EUR 811.20
  • EUR 326.40
  • EUR 2481.60
  • EUR 961.20
  • EUR 577.20
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Rat Troponin T Type 1, Slow Skeletal (TNNT1) Protein
20-abx069498
  • EUR 861.60
  • EUR 343.20
  • EUR 2665.20
  • EUR 1028.40
  • EUR 610.80
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Rat Actin Alpha 1, Skeletal Muscle (ACTA1) ELISA Kit
abx354231-96tests 96 tests
EUR 943.2

Rat Muscle Skeletal Receptor Tyrosine Kinase (MUSK) ELISA Kit
abx514524-96tests 96 tests
EUR 801.6

Rat Troponin T, Fast Skeletal Muscle (TNNT3) ELISA Kit
abx518123-96tests 96 tests
EUR 848.4

Rat Actin Alpha 1, Skeletal Muscle (ACTA1) ELISA Kit
abx571081-96tests 96 tests
EUR 943.2

Rat Troponin T, Slow Skeletal Muscle (TNNT1) ELISA Kit
abx575017-96tests 96 tests
EUR 801.6

Rat Troponin I, Fast Skeletal Muscle (TNNI2) ELISA Kit
abx575078-96tests 96 tests
EUR 801.6

Rat Troponin I, Slow Skeletal Muscle (TNNI1) ELISA Kit
abx575636-96tests 96 tests
EUR 801.6

Rat Troponin I, Fast Skeletal Muscle (TNNI2) ELISA Kit
abx256153-96tests 96 tests
EUR 895.2

Rat Troponin I, Slow Skeletal Muscle (TNNI1) ELISA Kit
abx256154-96tests 96 tests
EUR 895.2

Rat Troponin T, Fast Skeletal Muscle (TNNT3) ELISA Kit
abx256554-96tests 96 tests
EUR 848.4

Rat Troponin T, Slow Skeletal Muscle (TNNT1) ELISA Kit
abx256573-96tests 96 tests
EUR 848.4

Rat Muscle PrimaCell1: Normal Skeletal Muscle Cells Growth Medium
9-25073 5 x 100 ml Ask for price

Rat Troponin I, slow skeletal muscle(TNNI1) ELISA kit
CSB-EL024011RA-24T 1 plate of 24 wells
EUR 198
Description: Quantitativesandwich ELISA kit for measuring Rat Troponin I, slow skeletal muscle (TNNI1) in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Rat Troponin I, slow skeletal muscle(TNNI1) ELISA kit
1-CSB-EL024011RA
  • EUR 843.60
  • EUR 5811.60
  • EUR 3084.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Rat Troponin I, slow skeletal muscle(TNNI1) in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Rat Troponin I, fast skeletal muscle(TNNI2) ELISA kit
CSB-EL024012RA-24T 1 plate of 24 wells
EUR 198
Description: Quantitativesandwich ELISA kit for measuring Rat Troponin I, fast skeletal muscle (TNNI2) in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Rat Troponin I, fast skeletal muscle(TNNI2) ELISA kit
1-CSB-EL024012RA
  • EUR 964.80
  • EUR 6118.80
  • EUR 3244.80
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Rat Troponin I, fast skeletal muscle(TNNI2) in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Rat Troponin T, slow skeletal muscle(TNNT1) ELISA kit
CSB-EL024015RA-24T 1 plate of 24 wells
EUR 198
Description: Quantitativesandwich ELISA kit for measuring Rat Troponin T, slow skeletal muscle (TNNT1) in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Rat Troponin T, slow skeletal muscle(TNNT1) ELISA kit
1-CSB-EL024015RA
  • EUR 964.80
  • EUR 6118.80
  • EUR 3244.80
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Rat Troponin T, slow skeletal muscle(TNNT1) in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Rat Troponin T, fast skeletal muscle(TNNT3) ELISA kit
CSB-EL024017RA-24T 1 plate of 24 wells
EUR 198
Description: Quantitativesandwich ELISA kit for measuring Rat Troponin T, fast skeletal muscle (TNNT3) in samples from serum, plasma, cell culture supernates, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Rat Troponin T, fast skeletal muscle(TNNT3) ELISA kit
1-CSB-EL024017RA
  • EUR 964.80
  • EUR 6118.80
  • EUR 3244.80
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Rat Troponin T, fast skeletal muscle(TNNT3) in samples from serum, plasma, cell culture supernates, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Rat Actin Alpha 1, Skeletal Muscle (ACTa1) ELISA Kit
DLR-ACTa1-Ra-48T 48T
EUR 633.6
Description: A sandwich quantitative ELISA assay kit for detection of Rat Actin Alpha 1, Skeletal Muscle (ACTa1) in samples from tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Rat Actin Alpha 1, Skeletal Muscle (ACTa1) ELISA Kit
DLR-ACTa1-Ra-96T 96T
EUR 828
Description: A sandwich quantitative ELISA assay kit for detection of Rat Actin Alpha 1, Skeletal Muscle (ACTa1) in samples from tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Rat Tnni2(Troponin I, fast skeletal muscle) ELISA Kit
ER0168 96T
EUR 681.12
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Rattus;Sensitivity: 18.75pg/ml

Rat Tnni1(Troponin I, slow skeletal muscle) ELISA Kit
ER0169 96T
EUR 681.12
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Rattus;Sensitivity: 18.75pg/ml

Rat Tnnt3(Troponin T, fast skeletal muscle) ELISA Kit
ER0579 96T
EUR 628.92
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Rattus;Sensitivity: 18.75pg/ml

Rat Tnnt1(Troponin T, slow skeletal muscle) ELISA Kit
ER0598 96T
EUR 628.92
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Rattus;Sensitivity: 9.375pg/ml

ELISA kit for Rat RYR1 (Ryanodine Receptor 1, Skeletal)
ELK6558 1 plate of 96 wells
EUR 518.4
Description: A sandwich ELISA kit for detection of Ryanodine Receptor 1, Skeletal from Rat in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

Rat Troponin I, fast skeletal muscle(TNNI2) ELISA kit
E02T0719-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Rat Troponin I, fast skeletal muscle(TNNI2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Troponin I, fast skeletal muscle(TNNI2) ELISA kit
E02T0719-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Rat Troponin I, fast skeletal muscle(TNNI2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Troponin I, fast skeletal muscle(TNNI2) ELISA kit
E02T0719-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Rat Troponin I, fast skeletal muscle(TNNI2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Troponin T, fast skeletal muscle(TNNT3) ELISA kit
E02T0722-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Rat Troponin T, fast skeletal muscle(TNNT3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Troponin T, fast skeletal muscle(TNNT3) ELISA kit
E02T0722-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Rat Troponin T, fast skeletal muscle(TNNT3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Troponin T, fast skeletal muscle(TNNT3) ELISA kit
E02T0722-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Rat Troponin T, fast skeletal muscle(TNNT3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Tnni1/ Troponin I, slow skeletal muscle ELISA Kit
E0992Ra 1 Kit
EUR 666

Rat Tnni2/ Troponin I, fast skeletal muscle ELISA Kit
E0993Ra 1 Kit
EUR 666

Rat Tnnt1/ Troponin T, slow skeletal muscle ELISA Kit
E0995Ra 1 Kit
EUR 685.2

Rat Tnnt3/ Troponin T, fast skeletal muscle ELISA Kit
E0997Ra 1 Kit
EUR 685.2

ELISA kit for Rat Troponin T, fast skeletal muscle
EK3859 96 tests
EUR 663.6
Description: Enzyme-linked immunosorbent assay kit for quantification of Rat Troponin T, fast skeletal muscle in samples from serum, plasma, tissue homogenates and other biological fluids.

ELISA kit for Rat Troponin T, slow skeletal muscle
EK3993 96 tests
EUR 663.6
Description: Enzyme-linked immunosorbent assay kit for quantification of Rat Troponin T, slow skeletal muscle in samples from serum, plasma, tissue homogenates and other biological fluids.

ELISA kit for Rat Troponin I, slow skeletal muscle
EK4331 96 tests
EUR 582
Description: Enzyme-linked immunosorbent assay kit for quantification of Rat Troponin I, slow skeletal muscle in samples from serum, plasma, tissue homogenates and other biological fluids.

Rat Actin Alpha 1(Skeletal Muscle(ACTa1))ELISA Kit
GA-E0664RT-48T 48T
EUR 424.8

Rat Actin Alpha 1(Skeletal Muscle(ACTa1))ELISA Kit
GA-E0664RT-96T 96T
EUR 685.2

Rat Actin Alpha 1, Skeletal Muscle (ACTa1) ELISA Kit
RD-ACTa1-Ra-48Tests 48 Tests
EUR 640.8

Rat Actin Alpha 1, Skeletal Muscle (ACTa1) ELISA Kit
RD-ACTa1-Ra-96Tests 96 Tests
EUR 890.4

Rat Actin Alpha 1, Skeletal Muscle (ACTa1) ELISA Kit
RDR-ACTa1-Ra-48Tests 48 Tests
EUR 669.6

Rat Actin Alpha 1, Skeletal Muscle (ACTa1) ELISA Kit
RDR-ACTa1-Ra-96Tests 96 Tests
EUR 931.2

Rat Actin Alpha 1(Skeletal Muscle(ACTa1))ELISA Kit
QY-E10988 96T
EUR 433.2

Rat Myosin Heavy Chain 3, Skeletal Muscle, Embryonic (MYH3) Protein
20-abx068145
  • EUR 844.80
  • EUR 343.20
  • EUR 2581.20
  • EUR 994.80
  • EUR 594.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Rat Myosin Heavy Chain 8, Skeletal Muscle, Perinatal (MYH8) Protein
20-abx068153
  • EUR 811.20
  • EUR 326.40
  • EUR 2464.80
  • EUR 961.20
  • EUR 577.20
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
The evolutionary conservation and expression analyses of E. multilocularis miRNAs all through metacestode growth together with the in silico practical analyses of their predicted targets may assist to establish selective therapeutic targets for therapy and management of AE.
Tags :  , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , ,

Leave a Reply

Your email address will not be published. Required fields are marked *